ADP + Orthophosphate ( Pi ) Stimulates an Na / K Pump - mediated Coefflux of Pi and Na in Human Red Blood Cell
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چکیده
Dr. Marin's permanent address is Instituto Venezolano de Investigaciones Cientificas, CBB. Aptdo. 21827, Caracas 1020A, Venezuela. Address correspondence to Joseph F. Hoffman, Dept. of Cellular and Molecular Physiology, Yale University School of Medicine, 333 Cedar Street, New Haven, CT 06510. J. GEN. PHYSIOL. 9 The Rockefeller University Press 9 0022-1295/94/07/0033/23 $2.00 Volume 104 July 1994 33-55 33 on Jne 3, 2017 D ow nladed fom Published July 1, 1994
منابع مشابه
ADP+orthophosphate (P(i)) stimulates an Na/K pump-mediated coefflux of P(i) and Na in human red blood cell ghosts
The Na/K pump in human red blood cells that normally exchanges 3 Nai for 2 Ko is known to continue to transport Na in a ouabain-sensitive and ATP-dependent manner when the medium is made free of both Nao and Ko. Although this Na efflux is called "uncoupled" because of removal of ions to exchange with, the efflux has been shown to be comprised of a coefflux with cellular anions. The work describ...
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This paper is concerned with analyzing the sidedness of action of various determinants which alter the rate of ouabain binding to human red blood cell ghosts. Thus, ouabain binding promoted by orthophosphate (Pi) and its inhibition by Na are shown to be due to inside Pi and inside Na. External K inhibits Pi-promoted ouabain binding and Nao acts to decrease the effectiveness of Ko. Similarly, in...
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We have studied the interaction of physiological ligands other than Nai and Ca; with the Ca pump and Na/Ca exchange in internally dialyzed squid axons. The results show the following. (a) Internal Mg` is an inhibitor of the Na,,-dependent Ca efflux . At physiological me, (4 mM), the inhibition amounts to ~50%. The inhibition is partial and noncompetitive with Ca;, and is not affected by Nai or ...
متن کاملSodium-phosphate cotransport in human red blood cells. Kinetics and role in membrane metabolism
Orthophosphate (Pi) uptake was examined in human red blood cells at 37 degrees C in media containing physiological concentrations of Pi (1.0-1.5 mM). Cells were shown to transport Pi by a 4,4'-dinitro stilbene-2,2'-disulfonate (DNDS) -sensitive pathway (75%), a newly discovered sodium-phosphate (Na/Pi) cotransport pathway (20%), and a pathway linearly dependent on an extracellular phosphate con...
متن کاملInteractions of physiological ligands with the Ca pump and Na/Ca exchange in squid axons
We have studied the interaction of physiological ligands other than Nai and Cai with the Ca pump and Na/Ca exchange in internally dialyzed squid axons. The results show the following. (a) Internal Mg2+ is an inhibitor of the Nao-dependent Ca efflux. At physiological Mg2+i (4 mM), the inhibition amounts to approximately 50%. The inhibition is partial and noncompetitive with Cai, and is not affec...
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تاریخ انتشار 2003